Cloning and relative expression of oestrogen receptor isoforms ERα and ERβ in the Three-spined stickleback, Gasterosteus aculeatus
F.Geoghegan (1), Y. Katsu (2), I. Katsiadaki (3), T, Iguchi (2), and J. K. Chipman (1)
- University of Birmingham, UK
- Okazaki Institute for Integrative Bioscience, Japan
- Cefas, Weymouth laboratory, UK
Abstract - An approach used to elucidate mechanisms of response following exposure to endocrine disrupting compounds is to target genes that are thought to play a significant role. The aim of this collaborative project was therefore to use a PCR based method to clone and sequence oestrogen receptors in the three-spined stickleback and then use a real-time PCR assay to investigate relative expression levels in samples from fish that had been exposed to various compounds.
